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Short-term transplantation of isolated human ovarian follicles and cortical tissue into nude mice

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NCr Nude Mice | Athymic Mouse Model | Taconic Biosciences

The effects of antiestrogens, tamoxifen and ICI ,, and aromatase inhibitors, arimidex anastrozole ZD and letrozole CGS 20, , on the growth of tumors were studied in nude mice. In this model, estrogen dependent MCF-7 human breast cancer cells stably transfected with the aromatase gene were inoculated in four sites per mouse. Sufficient estrogen is produced from aromatization of androstenedione supplement 0. Once the tumors reached a measurable size, the mice were injected with antiestrogen or inhibitor for 35—56 days. Tumor volumes were measured weekly.
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Experimental endometriosis: the nude mouse as a xenographic host

In order to investigate the potential source of such iron deposits, we examined whether the seric and erythrocyte fractions of menstrual effluent might influence the occurrence of iron deposition within lesions in a murine model of endometriosis. METHODS: Endometriosis was induced in 57 nude mice by intraperitoneal injection of unfractionated human menstrual effluent, endometrial fragments plus serum, endometrial fragments plus erythrocytes or endometrial cell fraction alone. The number of implants, histologic aspect, proliferative activity and iron deposition in lesions was assessed. This may be one of the factors triggering oxidative damage and chronic inflammation. Endometriosis is a common gynaecologic disease characterized by the presence and growth of endometrial tissue outside the uterine cavity.
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This study was designed to evaluate follicular survival and growth after short-term transplantation of fresh isolated human follicles and ovarian cortical tissue to nude mice. Ovarian biopsies were obtained from nine women undergoing laparoscopy. Twelve nude mice were xenografted with an ovarian cortical fragment in the right ovarian bursa, and a clot containing isolated follicles in the left, for a period of 7 days. One ungrafted fragment was used as a control. Histological sections were analyzed to determine follicle number and stage.
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